Journal: PLoS ONE

Article Title: Pro-Inflammatory Action of MIF in Acute Myocardial Infarction via Activation of Peripheral Blood Mononuclear Cells

doi: 10.1371/journal.pone.0076206

Figure Lengend Snippet: A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or anti-MIF polyclonal antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.

Article Snippet: To investigate the effect of anti-MIF intervention on inflammatory responses, animals were treated with a single dose of anti-MIF polyclonal antibody (Santa Cruz, sc-20121) or isotype control rabbit IgG (Santa Cruz, sc-2027), at 5 mg/kg i.p. immediately after CAO.

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing

Journal: Journal of Dairy Science

Article Title: Evaluating the in vitro efficacy of bovine lactoferrin products against SARS-CoV-2 variants of concern

doi: 10.3168/jds.2021-21247

Figure Lengend Snippet: Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).

Article Snippet: Dose-response curves for this data were fitted in GraphPad Prism 9.0 (GraphPad Software) using a semi-log 4-parameter variable slope model.

Techniques: Activity Assay, Microscopy